Home > Liquid Chromatography (LC) and Mass Spectrometry (MS) Service

Liquid Chromatography (LC) and Mass Spectrometry (MS) Service

The service applies liquid chromatography (LC) and mass spectrometry (MS) for identification and quantification of non-polar molecules and low, medium or high polar molecular weight molecules.

The Laboratory is equipped with a Triple Quadrupole MS Micromass Quattro Micro API and a HPLC Waters Alliance 2695 coupled to a Photodiode Array Detector Waters 996 PDA.

The equipment was financed by FCT (Programa Nacional de Reequipamento Científico REDE/1518/REM/2005).

LC-MS characteristics:

  – ESI e APCI sources (m/z between 20-2040; MS/MS)
  – Liquid Chromatographer (Alliance, Waters 2695) with automatic sampler
  – PDA detector (Waters 2996)
  – Separations module(Collector III, Waters)
  – Software: Waters MassLynx version 4.1 for Quattro Micro API and Waters Empower
The LC-MS Service provides varied services:
 – Small molecules: Atmospheric Pressure Chemical Ionization (APCI)
 – Small or large non-volatile molecules: Electrospray Ionization (ESI)
 – Mixtures: Liquid Chromatography/ Mass Spectrometry (LC-MS/MS)