Coimbra, December 4th

Methods

09:00 Opening session

09:05 M1 Vitrification, first step for EM analysis of biological samples - C. López-Iglesias

10:00 M2 Cryo-methods for in situ molecular localization - C. López-Iglesias

11:00 Cofee break

11:30 M3 Immunocytochemical methods in Electron Microscopy - Graça Raposo

12:30 M4 Electron tomography and visualization of nanomachines - Peter Peters

13:30 Lunch

Aplications

15:00 A1 The endocytic pathway in Cell Biology and Pathology - Graça Raposo

16:00 A2 Dissecting virus replication and morphogenesis by Cryo methods - José Carrascosa

17:00 A3 Structure and Distribution of Prions - Peter Peters

18:00 Closing session

18:05 General Assembly of SPMicros

Coimbra, December 5th

Practical demonstrations (Peter Peters, Carmen López-Iglesias and Graça Raposo):

Cryo-fixation and Cryo-substitution

Cryo-Ultramicrotomy

Immunocytochemistry

Available equipment:

            TEM FEI-Tecnai G2 Spirit Biotwin

            Ultramicrotome for ultrathin low temperature sectioning - Leica EM UC6 + EM FC6

            High Pressure Freezer for cryofixation - Leica EM HPM100